How to identify hybrid cotton F1 cotton varieties?

November 04, 2023

The insect resistance basis of the transgenic insect-resistant cotton lies in that the insect-resistant cotton genetic material Bt insecticidal gene can be generated in the insect-resistant cotton plant, and the property of separating the plant genetic trait and the sensitivity of the Bt insecticidal gene to kanamycin sulfate can be To achieve the purpose of identifying hybrid insect-resistant cotton. One is to identify drugs, one with kanamycin sulfate; the second is to identify the time, generally in the four-leaf cotton to budding stage; three is to identify the site, select the top of the cotton plant fully expanded leaves; fourth is the identification method, selected sunny Use a pair of kanamycin sulfate to purify 0.5 kg of water. Then mix the solution with a brush and apply it to the identification site. If it rains within 6 hours after coating, it must be recoated. After 3-4 days, observe the color of the leaves coated with the mixture. Yellowing indicates that the cotton is not insect-resistant, and that it does not change yellow indicates that the cotton is an insect-resistant hybrid cotton. If the cotton whose leaves are not discolored accounts for more than 98% of the selected cotton plants, it can be regarded as a true hybrid F1 hybrid cotton. One of the differences between the F2 insect-resistant cotton and the F1 cotton is that 25%-30% of the cotton plants are not resistant to insects after separation of the F2 generation traits. The leaves of the cotton plants turn yellow in the case of kanamycin sulfate.

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